Matchmaker™ Library Construction & Screening Kits （pGBKT7，pGADT7，pGBKT7-53，pGADT7-T，pGBKT7-Lam）

发布时间：2013/7/23 点击次数：6338

pGBKT7-53

产品参数:

Bacteria Resistance：KanamycinMammalian Selection：-

载体抗性:

卡那霉素（Kanamycin）

载体描述:

载体特点：与pGADT7-T构成Matchmaker Two-Hybrid System 3的双杂交正对照。

pGBKT7-53 encodes a fusion of the murine p53 protein （a.a. 72–390） and the GAL4 DNA-BD （a.a. 1–147）. The murine p53 cDNA （GenBank Accession #K01700） was cloned into pGBKT7 at the EcoR I and BamH I sites. The p53 insert was derived from the plasmid described in Iwabuchi et al. （1993）; plasmid modification was performed at Clontech. pGBKT7-53 has not been sequenced.

质粒图谱:

pGBKT7-Lam

产品参数:

Bacteria Resistance：KanamycinMammalian Selection：-

载体抗性:

卡那霉素（Kanamycin）

载体描述:

载体特点：Matchmaker Two-Hybrid System 3的负对照。

pGBKT7-Lam is a negative control plasmid that encodes a fusionof the human lamin C protein （a.a. 66–230） and the GAL4 DNA-BD （a.a. 1–147）. The lamin C cDNA insert （GenBank Accession #M13451） was derived from the plasmid referenced in Bar et al. （1993a）. Plasmid modification was performed atBD Biosciences Clontech. Yeast cotransformed with pGBKT7-Lam and pGADT7-RecT, provide a measure of the backgroundthat is due to false-positive two-hybrid interactions. pGBKT7-Lam has not been sequenced.

质粒图谱:

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