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SYSY 131 002 说明书

更新时间:2017-08-09      点击次数:1910

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VGAT (vesicular GABA transporter, VIAAT)

GABA transporter in the membrane of synaptic vesicles

Fact Sheet Cat. No. 131 002

Polyclonal rabbit antiserum, antibody 1 cytoplasmic domain
Cat. No. 131 002
crude antiserum
200 µl antiserum, lyophilized. For reconstitution add 200 µl H2O, then aliquot and store at -20°C until use.
ApplicationsWB: 1 : 1000 up to 1 : 5000 (AP staining, see remarks) blot 
IP: yes 
ICC: 1 : 100 up to 1 : 1000 image 
IHC: 1 : 500 up to 1 : 3000 image 
IHC-P: yes
ImmunogenSynthetic peptide AEPPVEGDIHYQR (aa 75 - 87 in rat) coupled to key-hole limpet hemocyanin via an added N- terminal cysteine.
ReactivityHuman, rat, mouse, monkey; other species not tested yet.
SpecificitySpecific for VGAT (K.O. verified).
RemarksWB: VGAT aggregates after boiling, making it necessary to run SDS-PAGE only withnon-boiled samples.

 

VGAT(水泡GABA转运蛋白,VIAAT)

突触小泡膜中的GABA转运蛋白

一般信息

The vesicular GABA transporter VGAT is responsible for uptake and storage of GABA and glycine by synaptic vesicles in the central nervous system. For this reason it is frequently referred to as thevesicular inhibitory aminoacid transporter VIAAT. It is different from the plasma membrane transporters in that it is driven by a proton electrochemical gradient across the vesicle membrane. So far, only one isoform is known. VGAT is currently the best marker for inhibitory nerve terminals.

 

相关产品信息如下:

Cat. No.AntigenProductApplicationsCloneQty.
 
131 011VGAT 
cytoplasmic domain
mouse monoclonal, purified IgG, unlabeled 
K.O. verified
WB IP ICC IHC IHC-P EM117G4100µg
131 011C2VGAT 
cytoplasmic domain
mouse monoclonal, purified IgG, Oyster 488-labeled 
K.O. verified
ICC117G4100µg
131 011C3VGAT 
cytoplasmic domain
mouse monoclonal, purified IgG, Oyster 550-labeled 
K.O. verified
ICC IHC117G4100µg
131 011BTVGAT 
cytoplasmic domain
mouse monoclonal, purified IgG, biotin-labeled 
K.O. verified
WB IP ICC IHC117G4100µg
131 002VGAT 
cytoplasmic domain
rabbit polyclonal, antiserum 
antibody 1, K.O. verified
WB IP ICC IHC IHC-P 200µl
131 003VGAT 
cytoplasmic domain
rabbit polyclonal, affinity purified 
antibody 1, K.O. verified
WB IP ICC IHC EM 50µg
131 006VGAT 
cytoplasmic domain
chicken polyclonal, purified IgYICC IHC IHC-P 50µg
131-0PVGAT 
cytoplasmic domain
control peptide  100µg
131 013VGAT 
cytoplasmic domain
rabbit polyclonal, affinity purified 
antibody 2, K.O. verified
WB IP ICC IHC EM 50µg
131 004VGAT 
cytoplasmic domain
Guinea pig polyclonal, antiserum, K.O. verifiedWB ICC IHC IHC-P EM 100µl
131-0GPVGAT 
cytoplasmic domain
control protein  100µg
131 103VGAT 
lumenal domain
rabbit polyclonal, affinity purified, unlabeled 
K.O. verified
WB IP ICC IHC 50µg
131 103C2VGAT 
lumenal domain
rabbit polyclonal, affinity purified, Oyster 488-labeled 
K.O. verified
ICC IHC 50µg
131 103C3VGAT 
lumenal domain
rabbit polyclonal, affinity purified, Oyster 550-labeled 
K.O. verified
ICC IHC 50µg
131 103C5VGAT 
lumenal domain
rabbit polyclonal, affinity purified, Oyster 650-labeled 
K.O. verified
ICC IHC 50µg
131 103CpHVGAT 
lumenal domain
rabbit polyclonal, affinity purified, CypHer5E-labeled 
K.O. verified
ICC IHC 50µg

 

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