ࡱ; 54  "#$%&'()*+,-./01236Root Entry  FMicrosoft Word-Dokument MSWordDocWord.Document.89qOh+'0@H d p | Jingchao Yang Yang0@@@9m@՜.+,D՜.+, [\\؞$a$1$*$A$3B*OJQJCJsH sH KHPJnH^JaJ_H9tH9BA@BAbsatz-StandardschriftartBBAbsatz-StandardschriftartSW[&{<"<h x$OJ QJ CJPJ ^JaJ B" cke x/!2Rh^J 6B6l xx $CJ6^J aJ] R vU_ $^J J$ :      ^`.^8`8.^`.^`.^p`p.^ ` .^@ `@ .^ ` .^`.^`.^8`8.^`.^`.^p`p.^ ` .^@ `@ .^ ` .^`.^`.^8`8.^`.^`.^p`p.^ ` .^@ `@ .^ ` .^`.^`.^8`8.^`.^`.^p`p.^ ` .^@ `@ .^ ` .^`.^`.^8`8.^`.^`.^p`p.^ ` .^@ `@ .^ ` .^`.^`.^8`8.^`.^`.^p`p.^ ` .^@ `@ .^ ` .^`.^`.^8`8.^`.^`.^p`p.^ ` .^@ `@ .^ ` .^`.^`.^8`8.^`.^`.^p`p.^ ` .^@ `@ .^ ` .^`.^`.^8`8.^`.^`.^p`p.^ ` .^@ `@ .^ ` .^`.^`.^8`8.^`.^`.^p`p.^ ` .^@ `@ .^ ` .^`.^`.^8`8.^`.^`.^p`p.^ ` .^@ `@ .^ ` .^`.^`.^8`8.^`.^`.^p`p.^ ` .^@ `@ .^ ` .^`.^`.^8`8.^`.^`.^p`p.^ ` .^@ `@ .^ ` .^`.^`.^8`8.^`.^`.^p`p.^ ` .^@ `@ .^ ` .^`.^`.^8`8.^`.^`.^p`p.^ ` .^@ `@ .^ ` .^`.^`.^8`8.^`.^`.^p`p.^ ` .^@ `@ .^ ` .^`.^`.^8`8.^`.^`.^p`p.^ ` .^@ `@ .^ ` .^`.^`.^8`8.^`.^`.^p`p.^ ` .^@ `@ .^ ` .^`. ^`.^8`8.^`.^`.^p`p.^ ` .^@ `@ .^ ` .^`. ^`.^8`8.^`.^`.^p`p.^ ` .^@ `@ .^ ` .^`.^`.^8`8.^`.^`.^p`p.^ ` .^@ `@ .^ ` .^`.^`.^8`8.^`.^`.^p`p.^ ` .^@ `@ .^ ` .^`.^`.^8`8.^`.^`.^p`p.^ ` .^@ `@ .^ ` .^`.^`P^@`@^`0^```^`^`^`^``^0`0 WW8Num1WW8Num2WW8Num3WW8Num4WW8Num5WW8Num6WW8Num7WW8Num8WW8Num9WW8Num10WW8Num11WW8Num12WW8Num13WW8Num14WW8Num15WW8Num16WW8Num17WW8Num18WW8Num19WW8Num20WW8Num21WW8Num22WW8Num23JJP GTimes New Roman5Symbol3&ArialGTimes New Romana$Univers-CondensedBoldOblique3&ArialS$Univers-CondensedBold-[SO5Mangal3&Arial1_oŖў5MangalBhF;fc<c<' 0 0M 0Caolan80 2$J 4N Z$225b2~,/12 Santa Cruz Biotechnology TECHNICAL SERVICE GUIDE: Western Blotting Catalog #: Lot #: PROBLEM AND PREVIOUS EXPERIENCE What is the specific problem you are experiencing? What size bands were expected and what size bands were detected? Was the blot blank or was a dark background or non-specific bands seen? Did this same vial of product work in the past? What were the results? Did other lots of this product work in the past? Which lots? What were the results? SAMPLES AND CONTROLS From what species (animal) was the sample and what type of sample was used (cell lysate, tissue, purified protein, etc.)? What lysis buffer was used? How much sample was loaded on the gel? If the blocking peptide was used, was it used as a negative control? What positive and/or negative controls were used, and what were the results? BLOCKING What were the blocking conditions (blocking solution, how long, what temperature. Etc.)? PRIMARY AND SECONDARY ANTIBODIES At what dilution(s) was the primary antibody used and what was the diluent? What were the incubation conditions (time, temperature) for the primary antibody? From which company was the secondary antibody obtained and what type of secondary was being used (HRP or AP conjugated, anti-rabbit or anti-goat, etc.)? What detection system was used, and was it compatible with the secondary conjugate? At what dilution(s) was the secondary antibody used and what was the diluent? What were the incubation conditions (time, temperature) for the secondary antibody? Has a secondary control been performed successfully? If using Cruz Markers, how many L were loaded, and was a Cruz Marker compatible secondary antibody used? Can you verify that all Molecular Weight Markers transferred to the membrane? At what temperature has the primary antibody been stored? Has it ever been frozen? MEMBRANE, GEL, DETECTION What detection system was used and what were the exposure times for the film? What type of membrane was used (PVDF or nitrocellulose)? What percentage gel was used? Was this appropriate for the size of the protein? 2& (   4 6 8 : xzVX02xz"$df|~ǴǴǴǴǴǴǴǴǴǴǴǴǴǴǴǴǴǴǴǴǴǴǴǴǴǴǴǴ$CJ65PJ^JaJ]\OJQJ$CJ65PJ^JaJ]\OJQJ$CJ65PJ^JaJ]\OJQJ$CJ65PJ^JaJ]\OJQJ=2& (   4 6 8 : & F$a$7$ & F$a$7$ & F$a$7$ & F$a$7$ & F$a$7$ & F$a$7$$a$7$: xzVX02 & F$a$7$ & F $a$7$ & F $a$7$ & F $a$7$ & F $a$7$ & F $a$7$ & F$a$7$$a$7$ & F$a$7$xz"$df|~ & F$a$7$ & F$a$7$ & F$a$7$ & F$a$7$ & F$a$7$ & F$a$7$ & F$a$7$ & F$a$7$$a$7$$a$7$ & F$a$7$0/ =!n"n#n$n2P1h0p3P(20\Root Entry F@CompObjjOle 1Table8SummaryInformation(WordDocument!2$DocumentSummaryInformation8t